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ermine the optimal conditions for the reaction. Catalysis resulted to be metal-dependent (Figure 3A). Among the tested divalent cations, Co+2 was the most effective in supporting the enzyme activity, with Ni+2, Mg+2 and Mn+2 being about seven-fold less efficient; 1 mM Ca+2, Cu+2 and Zn+2 did not sustain the activity at all (Figure 3A). Mg+2 and Co+2 titration experiments showed that the enzyme-cat
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